[Abstract]

Coronary artery disease refers to coronary vessel occlusion, leading to myocardial ischemia, hypoxia caused by heart disease, coronary artery functional changes in it and referred to coronary heart disease, referred to as coronary heart disease, also known as lack of congestive heart disease. Coronary heart disease is the result of atherosclerosis of the most common type of organ. Many diseases have multiple causes due to abnormal apoptosis cells, such as cancer, nervous system, immune and cardiovascular systems. To cardiovascular diseases, and clinical studies confirmed in vitro: Cell apoptosis and abnormal atherosclerosis, hypertension, cardiomyopathy, arrhythmia and heart failure and other diseases are related. With the development of cardiovascular molecular biology, a new independent risk factor for coronary heart disease: homocysteine HCY) hyperlipidemia, receive more attention. Experimental results demonstrate endothelial cells induced by homocysteine induce apoptosis, thus contributing to the occurrence of atherosclerosis development, its mechanism has gradually clear.Chinese Sedum Rhodiola Rhodiola, experimental studies and observations suggest that: Rhodiola on cardiovascular and cerebrovascular diseases have a significant effect, can prevent and treat cardiovascular disease, menopausal syndrome, and the resulting instability angina, precordial men Tong, heart palpitations, shortness of breath symptoms. Salidroside and its aglycone tyrosol, casein Savi, Rhodiola is an effective pharmacological factors such as active ingredients.Research proved homocysteine can induce apoptosis of endothelial cells, leading to the progress of atherosclerosis, salidroside is proven on cardiovascular and cerebrovascular disease prevention and treatment has a significant effect. The focus of this study is to discuss the role of salidroside induced by homocysteine in endothelial cells after the impact, not only for the study of atherosclerosis provide a clue to the pathogenesis, but also for the prevention of atherosclerosis and treatment also has some significance for Integrative Medicine in the treatment of cardiovascular disease provide new ideas, new ideas. Objective salidroside on homocysteine induced endothelial cell apoptosisMethod:1. To in vitro endothelial cell line as experimental subjects, 37℃environment training, the establishment of apoptotic cell model, inverted microscope, MTT was observed by different concentrations of homocysteine, HUVEC induced by different forms of time, the survival activity impact; application flow cytometry (FCM) detection of HUVEC apoptosis; Application Western blot detection of homocysteine on apoptosis-related protein Caspase-3, Bax and Bcl-2 transcription in HUVEC cells and expression;2. To in vitro endothelial cell line as experimental subjects, 37℃environment training, the establishment of intervention salidroside apoptosis model, inverted microscope, MTT salidroside observed by different concentrations, has been acting on different time Homocysteine-induced HUVEC morphology, vigor of life; application of flow cytometry (FCM) detection of HUVEC apoptosis; application of Western blot detection of apoptosis-related protein Caspase-3, Bax and Bcl-2 transcription in HUVEC cells and expression;Results:1. After intervention salidroside HUVEC 24h, some cells detached from the attachment and growth in suspension, adherent diminished capacity, the cells became round and smaller in size, enhance the refraction.2. MTT assay showed that homocysteine significantly suppressed the proliferation of HUVEC cells.3. FCM assay showed that homocysteine significantly induced G1 phase retardation and apoptosis of HUVEC cells. 4. Western blot analysis showed that increased expression of caspase-3 and Bax, decreased expression of Bcl-2 in HUVEC cells under homocysteine stimulation.5. Application of homocysteine along with Salidroside could suppress all of the above effects of homocysteine.Conclusion: We conclude that homocysteine on HUVEC cells with distinct morphological changes, can inhibit the growth and proliferation of HUVEC, and vascular endothelial cells. Salidroside role in the induced reversible morphogenesis of HUVEC cells can change to inhibit HUVEC apoptosis is inhibited apoptosis of human umbilical vein endothelial cells one of the main mechanisms. Further suggesting that it may inhibit the occurrence of atherosclerosis or the development of an important role, not only to study the pathogenesis of coronary heart disease provides further clues, and the prevention and treatment of coronary heart disease is important.

Title: Inhibition Effects of Salidroside on Homocysteine-induced HUVEC Cell Apoptosis

Category: Tumor Stem Cell

Filename: Inhibition Effects of Salidroside on Homocysteine-induced HUVEC Cell Apoptosis.pdf

Pages: 146

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HUVEC(Human Umbilical Vein Endothelial Cells) is vascular endothelial cells which is derived from umbilical vein with collagenase digestion.

HUVEC(Human Umbilical Vein Endothelial Cells) is with high purity, activity, and it can be passaged in vitro and the characteristics of more than seven generations. By flow cytometry cell surface markers (CD31, CD45, CD62, vWF) and low-density lipoprotein (LDL) phagocytosis assay cell purity, with propidium iodide to detect cell activity, cell purity and activity, respectively can reach 95 % and 90%.

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